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Novel assay to measure the plasmid mobilizing potential of mixed microbial communities Uli KlÃ¼mper, Ariadni Droumpali, Arnaud Dechesne & Barth F. Smets; Frontiers in Long-term manure exposure increases soil bacterial community potential for plasmid uptake. Environ Microbiol Rep, 6(2), 125-130. Abstract. Author URL. Klümper U, Droumpali A, Dechesne A, Smets BF (2014). Novel assay to measure the plasmid mobilizing potential of mixed microbial communities.

Novel assay to measure the plasmid mobilizing potential of mixed microbial communities

We compare the transfer frequency of a mobilizable plasmid to that of a mobilizing and conjugal plasmid measured for a model strain and for the assayed community. Frontiers in Journal Methods 25.11.2014 1 Novel assay to measure the plasmid mobilizing potential of mixed 2 microbial communities 3 4 Uli Klümper1, Ariadni Droumpali1, Arnaud Dechesne1, Barth F Novel assay to measure the plasmid mobilizing potential of mixed microbial communities By Uli KlÃ¼mper, Ariadni Droumpali, Arnaud Dechesne and Barth F. Smets Download PDF (3 MB) Novel assay to measure the plasmid mobilizing potential of mixed microbial communities By Uli KlÃ¼mper, Ariadni Droumpali, Arnaud Dechesne and Barth F. Smets Cite Novel assay to measure the plasmid mobilizing potential of mixed microbial communities . By Bahl, Barth, Boe-Hansen, Buchanan-Wollaston, Dechesne, Dubey, Novel assay to measure the plasmid mobilizing potential of mixed microbial communities By Barth Smets , Arnaud Dechesne , and Uli Klümper An individual-based approach to explain plasmid invasion in bacterial populations Here, we present a novel framework and experimental method to estimate the mobilization potential of mixed communities. We compare the transfer frequency of a mobilizable plasmid to that of a mobilizing and conjugal plasmid measured for a model strain and for the assayed community.

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Frontiers in Journal Methods 25.11.2014 1 Novel assay to measure the plasmid mobilizing potential of mixed 2 microbial communities 3 4 Uli Klümper1, Ariadni Droumpali1, Arnaud Dechesne1, Barth F We thank L. Riber and S. J. Sørensen for access to the tagged RSF1010 plasmid, L. K. Jensen for technical assistance in the laboratory and S. M. Milani for assistance in FACS sorting. This work was funded by the Villum Kann Rasmussen Foundation Center of Excellence CREAM (Center for Environmental and Agricultural Microbiology). Novel assay to measure the plasmid mobilizing potential of mixed microbial communities. Uli Klümper, Ariadni Droumpali, Arnaud Dechesne, Barth F. Smets.

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Novel assay to measure the plasmid mobilizing potential of mixed microbial communities

For transfer by conjugation, the TOP10 strain (Life Technologies, USA) was used as donor strain due to its high competence and leucine auxotrophy. 2016-09-23 2019-08-28 2018-03-07 Wastewater treatment plants (WWTPs) have been suggested as reservoirs and sources of antibiotic resistance genes (ARGs) in the environment. In a WWTP ecosystem, human enteric and environmental bacteria are mixed and exposed to pharmaceutical residues, potentially favoring genetic exchange and thus ARG transmission.

93 94 Here, we present a novel experimental method to estimate the plasmid mobilization potential of a 95 mixed bacterial community, using IncQ RSF1010 as model plasmid. Novel assay to measure the plasmid mobilizing potential of mixed microbial communities. December 2014; Frontiers in Microbiology 5(2) DOI: 10.3389/fmicb.2014.00730. Novel assay to measure the plasmid mobilizing potential of mixed microbial communities Uli Klümper*, Ariadni Droumpali, Arnaud Dechesne and Barth F. Smets* Department of Environmental Engineering,Technical University of Denmark, Kongens Lyngby, Denmark Edited by: Kornelia Smalla, Julius Kühn-Institut, Germany Reviewed by: Frontiers in Journal Methods 25.11.2014 1 Novel assay to measure the plasmid mobilizing potential of mixed 2 microbial communities 3 4 Uli Klümper1, Ariadni Droumpali1, Arnaud Dechesne1, Barth F Here, we present a novel framework and experimental method to estimate the mobilization potential of mixed communities.
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Front. Berg C, Gyllenhammar I and Kvarnryd M (2009) Xenopus tropicalis as a test An experimental pipeline for initial characterization of bacterial type III secretion system inhibitor i: Frontiers in Cellular and Infection Microbiology, E-ISSN 2235-2988, Vol. In situ plankton community respiration measurements show low respiratory Wild Birds in Live Birds Markets: Potential Reservoirs of Enzootic Avian additional genetic and post-transcriptional analyses reveals novel regulators of plasma Taxonomy of the Sorbus arranensis group (Rosaceae) in Norway, a hybrid fields and their local potentials2006Ingår i: Journal of Geometry and Physics, Community-wide plasmid gene mobilization and selection2013Ingår i: The 7 juli 2020 — (B) Plasmid pJNW684 består av en Himar1 mariner transposon (grön) 10 μL 10x hög-fidelity DNA-polymeras reaktion mix Qubit dsDNA HS Assay Kit, Thermo Fisher, Q32851 Vaara, M. Polymyxins and their novel derivatives. Acinetobacter baumannii Genes Required for Bacterial Survival during 16 mars 2017 — P-värdet beräknades med användning av ett oparat t-test.

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Novel assay to measure the plasmid mobilizing potential of mixed microbial communities. Front Microbiol, 5 Abstract. Author URL. Full text.

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